In-Vitro Growth of Vanda Tricolor Orchid Plantlets of Suavis Variety on ZPT IBA and Activated Charcoal Treatments

Puji, Astuti and Marisi, Napitupulu and Jumani, Jumani and Sari, Putri (2022) In-Vitro Growth of Vanda Tricolor Orchid Plantlets of Suavis Variety on ZPT IBA and Activated Charcoal Treatments. Jurnal BEPLS. pp. 102-107. ISSN 2277-1808

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Abstract

The aim of this research was to examine the effect of ZPT IBA and activated charcoal on plantula growth of Vanda tricolor orchid suavis variety on MS basic media, and to obtain the best concentration of IBA and activated charcoal from MS basic media treatment for plantula growth on Vanda tricolor orchid suavis variety. This research was done in the Tissue Culture Laboratory, Faculty of Agriculture Faculty, 17 Agustus 1945 Samarinda University from February until April 2019. This research used factorial experiment 4x4 which arranged in Randomized Complete Design. The Factor of ZPT IBA (I) which were consist of 4 levels, namely without ZPT IBA (i0); 0,3 ppm L-1 media (i1); 0,6 ppm L-1 media (i2), and 0,9 ppm L-1 media (i3). The factor of activated charcoal (A) consisting of 4 levels: without activated charcoal (a0); 2 g L-1 media (a1); 4 g L- 1 media (a2); and 6 g L-1 media (a3). The stages of research activities are as follows: (1) preparation of plant materials, (2) preparation of tools, (3) sterilization of equipment; (4) making media stock; (5) preparation of treatment media (in the form of MSO media as a control and media treated with IBA and activated charcoal); (6) plantula planting; (7) plantula maintenance, (8) data collection and analysis, and (9) reporting. The result a of research showed that : (1) the factor of IBA growth regulator affected significantly on root length at 3 months after planting, but no affected significantly on the increase in height and number of leaves at 1, 2 and 3 months, and the increase in the number of roots at age 3 months after planting the Vanda tricolor Orchid plantlet Varieties Suavis; (2) the factor of activated charcoal affected significantly on the increase in height 1 month after planting, but no affected significant on the increase in plant height 2 and 3 months, the increase in the number of leaves 1.2 and 3 months, the increase in the number of roots and length roots at the age of 3 months after planting the plantlet Vanda tricolor Orchid suavis variety; and (3) there was an interaction between ZPT IBA and activated charcoal treatment in the increase in height at 2 months, the number of leaves at 3 months, and the increase in the number of roots at 3 months after planting, but there was no interaction between the two treatment factors on increase in height at 1 and 3 months, increase in the number of leaves at 1 and 2 months, and increase in root length at 3 months after planting plantlet Vanda tricolor Orchid Suavis variety. Keywords: IBA, Activated Charcoal, Plantlet, Vanda tricolor

Item Type:	Article
Uncontrolled Keywords:	IBA, Activated Charcoal, Plantlet, Vanda tricolor
Subjects:	S Agriculture > S Agriculture (General)
Divisions:	Fakultas Pertanian > Program Studi Agroteknologi
Depositing User:	Unnamed user with email indah@untag-smd.ac.id
Date Deposited:	22 Dec 2023 05:34
Last Modified:	22 Dec 2023 05:34
URI:	http://repository.untag-smd.ac.id/id/eprint/433

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